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KMID : 0604520210470020139
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Journal of the Society of Cosmetic Scientists of Korea
2021 Volume.47 No. 2 p.139 ~ p.145
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Study on Reinforcing Skin Barrier and Anti-aging of Exosome-like Nanovesicles Isolated from Malus domestica Fruit Callus
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Seo Yu-Ri
Lee Kwang-Soo
Kang Yong-Won
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Abstract
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Plant-derived exosome-like nanovesicles (PELNs) are known to include various biological activities and possess high biocompatibility. Because PELNs can influence immune responses, cell differentiation, and proliferation regulation, they can be applied in multiple industries. However, the studies on the skin physiological of exosome-like nanovesicles derived from plant callus are insignificant compared to nanovesicles derived from mammalian cells. In this study, callus was induced from apple fruit (Malus domestica), and exosome-like nanovesicles (ACELNs) were isolated for improving skin barrier and anti-aging. The yield of ACELNs was 6.42 × 109 particles/mL, and the particle size was ranged from 100 to 200 nm. HDF cells and HaCaT cells were concentration-dependent, increased in cell, and decreased in cytotoxicity. The cornified envelope formation was significantly increased compared to the control group. The COL1A1 expression and the FBN1 expression in HDF cells were increased. In addition, the ACELNs promoted collagen biosynthesis in UVA-irradiated HDF cells. These results might be considered as potential materials that could improve skin barrier and prevent skin aging.
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KEYWORD
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plant callus, exosome-like nanovesicles, cosmetic, skin barrier, skin aging
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